A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities

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A multipurpose immobilized biocatalyst with pectinase, xylanase and cellulase activities

BACKGROUND The use of immobilized enzymes for catalyzing various biotransformations is now a widely used approach. In recent years, cross-linked enzyme aggregates (CLEAs) have emerged as a novel and versatile biocatalyst design. The present work deals with the preparation of a CLEA from a commercial preparation, Pectinex Ultra SP-L, which contains pectinase, xylanase and cellulase activities. T...

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Optimization of the Cellulase Free Xylanase Production by Immobilized Bacillus Pumilus

Background: The extracellular xylanase secreted by microorganisms is a hydrolytic enzyme, which arbitrarily cleaves the β-1, 4 backbone of the polysaccharide xylan; an enzyme used in the food processing, bio-pulping and bio-bleaching. The commercial production of the xylanase is limited because of a higher cost involvement, which can be overcome by the cost-effective production...

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Changes in cellulase and pectinase activities in fruit tissues and separation zones of citrus treated with cycloheximide.

Cellulase activity increased in separation-zone tissues 1 day after "Valencia" orange (Citrus sinensis [L.] Osbeck) was treated with 20 micrograms per milliliter cycloheximide. Exocellulase was detected only in the separation zones of treated fruit, whereas endocellulase was present in zones from both treated and control fruit. Endocellulase activity in separation-zone tissue of treated fruit w...

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Xylanase and cellulase activities during anaerobic decomposition of three aquatic macrophytes

Enzymatic activity during decomposition is extremely important to hydrolyze molecules that are assimilated by microorganisms. During aquatic macrophytes decomposition, enzymes act mainly in the breakdown of lignocellulolytic matrix fibers (i.e. cellulose, hemicellulose and lignin) that encompass the refractory fraction from organic matter. Considering the importance of enzymatic activities role...

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Hydrolytic activity of free and immobilized cellulase.

Cellulase is an enzymatic complex which synergically promotes the degradation of cellulose to glucose. The adsorption behavior of cellulase from Trichoderma reesei onto Si wafers or amino-terminated surfaces was investigated by means of ellipsometry and atomic force microscopy (AFM) as a function of temperature. Upon increasing temperature from (24 +/- 1) to (60 +/- 1) degrees C, adsorption of ...

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ژورنال

عنوان ژورنال: Chemistry Central Journal

سال: 2007

ISSN: 1752-153X

DOI: 10.1186/1752-153x-1-16